The original tracking macro written for ImageJ is called MTrack2, and was written by Nico Stuurman, of the Vale lab, at UCSF. It was developed by the NIH and is free to use. ![]() The tracking program that allows you to label each worm and track each worm's movements over the course of the video is called ImageJ. We are currently also looking at the possibility of shooting the videos in. It seems to work pretty well in the trials we have done. This software has not been field tested as extensively as the miXscope software has in the Mac format. Currently, we are recommending Webcam Surveyor. Therefore, if using a PC, it is necessary to install some sort of video capture software, in addition to the Digital Blue software. ![]() tiff formats and the Digital Blue software does not record time lapse in this format. ImageJ reads stacks of still images, shot as time lapse, in the. However, in the curriculum presented here, the worms are tracked using ImageJ software from the NIH. For most types of digital microscopy on a PC, the QX5 software is sufficient. The software for the QX5 is written for PC only. ![]() On a Mac, the miXscope software will power and control the microscope and do the video capture. All digital microscopy described in the lessons on the curriculum page utilize the QX5 Digital Blue microscope.
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